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Global changes in melanoma cells transcriptome following the knockdown (KD) of CCT3 . (A) Hierarchical cluster analysis of differentially expressed genes with fold change of > 2.0. Row and column represent gene and experimental cells, respectively. Upregulated and downregulated genes are shown in red and green, respectively. (B) Signaling enrichment analysis of CCT3-downregulated classical signaling pathways based on IPA. Differential data are presented as z-scores (based on Fold change). (C) The expression trends of molecules in cyclins and cell cycle regulation pathway (based on IPA). Red and green represent upregulated and downregulated genes, respectively. (D) Knowledge-based interaction network of CCT3 targets after comparing the CCT3-KD and the shCtrl cells. The network was built on the basis of the CCT3 interactome of <t>microarray</t> data with a 1.5-fold change cutoff. The intensity of the node color indicates the degree of upregulation (red) or downregulation (green). Light colors represent less significant p values.
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Global changes in melanoma cells transcriptome following the knockdown (KD) of CCT3 . (A) Hierarchical cluster analysis of differentially expressed genes with fold change of > 2.0. Row and column represent gene and experimental cells, respectively. Upregulated and downregulated genes are shown in red and green, respectively. (B) Signaling enrichment analysis of CCT3-downregulated classical signaling pathways based on IPA. Differential data are presented as z-scores (based on Fold change). (C) The expression trends of molecules in cyclins and cell cycle regulation pathway (based on IPA). Red and green represent upregulated and downregulated genes, respectively. (D) Knowledge-based interaction network of CCT3 targets after comparing the CCT3-KD and the shCtrl cells. The network was built on the basis of the CCT3 interactome of <t>microarray</t> data with a 1.5-fold change cutoff. The intensity of the node color indicates the degree of upregulation (red) or downregulation (green). Light colors represent less significant p values.
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Global changes in melanoma cells transcriptome following the knockdown (KD) of CCT3 . (A) Hierarchical cluster analysis of differentially expressed genes with fold change of > 2.0. Row and column represent gene and experimental cells, respectively. Upregulated and downregulated genes are shown in red and green, respectively. (B) Signaling enrichment analysis of CCT3-downregulated classical signaling pathways based on IPA. Differential data are presented as z-scores (based on Fold change). (C) The expression trends of molecules in cyclins and cell cycle regulation pathway (based on IPA). Red and green represent upregulated and downregulated genes, respectively. (D) Knowledge-based interaction network of CCT3 targets after comparing the CCT3-KD and the shCtrl cells. The network was built on the basis of the CCT3 interactome of <t>microarray</t> data with a 1.5-fold change cutoff. The intensity of the node color indicates the degree of upregulation (red) or downregulation (green). Light colors represent less significant p values.
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SOX2 and MMP-3 immunoreactivity in patient and xenograft melanomas. In patients (a–f), SOX2 (brown) and MMP-3 (blue) immunopositivity is located in small nests (c, d) or single cells (e, f) infiltrating stroma, in contrast to tumors with non-invasive perimeters (a, b). Adjacent cores from tissue <t>microarray</t> <t>(TMA)</t> (g, h) show strong SOX2/MMP-3 co-expression in small nests infiltrating stroma (g) and weaker immunostaining in expansile tumorigenic zones without stroma (h). Xenograft melanomas demonstrate preferential co-expression of SOX2 (brown by IHC, red by IF) and MMP-3 (blue by IHC, green by IF) along tumor edge (i, k; dotted), with a decrease toward tumor center (j, l; nuclei blue DAPI label). Xenografts from SOX2-KD cell line (m–p) were relatively devoid of SOX2 and MMP-3. (a–f, i–p) Bar = 50 μm, (g, h) bar = 500 μm.
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SOX2 and MMP-3 immunoreactivity in patient and xenograft melanomas. In patients (a–f), SOX2 (brown) and MMP-3 (blue) immunopositivity is located in small nests (c, d) or single cells (e, f) infiltrating stroma, in contrast to tumors with non-invasive perimeters (a, b). Adjacent cores from tissue <t>microarray</t> <t>(TMA)</t> (g, h) show strong SOX2/MMP-3 co-expression in small nests infiltrating stroma (g) and weaker immunostaining in expansile tumorigenic zones without stroma (h). Xenograft melanomas demonstrate preferential co-expression of SOX2 (brown by IHC, red by IF) and MMP-3 (blue by IHC, green by IF) along tumor edge (i, k; dotted), with a decrease toward tumor center (j, l; nuclei blue DAPI label). Xenografts from SOX2-KD cell line (m–p) were relatively devoid of SOX2 and MMP-3. (a–f, i–p) Bar = 50 μm, (g, h) bar = 500 μm.
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Image Search Results


Global changes in melanoma cells transcriptome following the knockdown (KD) of CCT3 . (A) Hierarchical cluster analysis of differentially expressed genes with fold change of > 2.0. Row and column represent gene and experimental cells, respectively. Upregulated and downregulated genes are shown in red and green, respectively. (B) Signaling enrichment analysis of CCT3-downregulated classical signaling pathways based on IPA. Differential data are presented as z-scores (based on Fold change). (C) The expression trends of molecules in cyclins and cell cycle regulation pathway (based on IPA). Red and green represent upregulated and downregulated genes, respectively. (D) Knowledge-based interaction network of CCT3 targets after comparing the CCT3-KD and the shCtrl cells. The network was built on the basis of the CCT3 interactome of microarray data with a 1.5-fold change cutoff. The intensity of the node color indicates the degree of upregulation (red) or downregulation (green). Light colors represent less significant p values.

Journal: Journal of Cancer

Article Title: Suppression of CCT3 inhibits melanoma cell proliferation by downregulating CDK1 expression

doi: 10.7150/jca.69497

Figure Lengend Snippet: Global changes in melanoma cells transcriptome following the knockdown (KD) of CCT3 . (A) Hierarchical cluster analysis of differentially expressed genes with fold change of > 2.0. Row and column represent gene and experimental cells, respectively. Upregulated and downregulated genes are shown in red and green, respectively. (B) Signaling enrichment analysis of CCT3-downregulated classical signaling pathways based on IPA. Differential data are presented as z-scores (based on Fold change). (C) The expression trends of molecules in cyclins and cell cycle regulation pathway (based on IPA). Red and green represent upregulated and downregulated genes, respectively. (D) Knowledge-based interaction network of CCT3 targets after comparing the CCT3-KD and the shCtrl cells. The network was built on the basis of the CCT3 interactome of microarray data with a 1.5-fold change cutoff. The intensity of the node color indicates the degree of upregulation (red) or downregulation (green). Light colors represent less significant p values.

Article Snippet: A human melanoma tissue microarray (TMA-ME803a) that contains 40 patient samples was purchased from Genechem Co.,Ltd.

Techniques: Knockdown, Protein-Protein interactions, Expressing, Microarray

SOX2 and MMP-3 immunoreactivity in patient and xenograft melanomas. In patients (a–f), SOX2 (brown) and MMP-3 (blue) immunopositivity is located in small nests (c, d) or single cells (e, f) infiltrating stroma, in contrast to tumors with non-invasive perimeters (a, b). Adjacent cores from tissue microarray (TMA) (g, h) show strong SOX2/MMP-3 co-expression in small nests infiltrating stroma (g) and weaker immunostaining in expansile tumorigenic zones without stroma (h). Xenograft melanomas demonstrate preferential co-expression of SOX2 (brown by IHC, red by IF) and MMP-3 (blue by IHC, green by IF) along tumor edge (i, k; dotted), with a decrease toward tumor center (j, l; nuclei blue DAPI label). Xenografts from SOX2-KD cell line (m–p) were relatively devoid of SOX2 and MMP-3. (a–f, i–p) Bar = 50 μm, (g, h) bar = 500 μm.

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: SOX2 contributes to melanoma cell invasion

doi: 10.1038/labinvest.2011.188

Figure Lengend Snippet: SOX2 and MMP-3 immunoreactivity in patient and xenograft melanomas. In patients (a–f), SOX2 (brown) and MMP-3 (blue) immunopositivity is located in small nests (c, d) or single cells (e, f) infiltrating stroma, in contrast to tumors with non-invasive perimeters (a, b). Adjacent cores from tissue microarray (TMA) (g, h) show strong SOX2/MMP-3 co-expression in small nests infiltrating stroma (g) and weaker immunostaining in expansile tumorigenic zones without stroma (h). Xenograft melanomas demonstrate preferential co-expression of SOX2 (brown by IHC, red by IF) and MMP-3 (blue by IHC, green by IF) along tumor edge (i, k; dotted), with a decrease toward tumor center (j, l; nuclei blue DAPI label). Xenografts from SOX2-KD cell line (m–p) were relatively devoid of SOX2 and MMP-3. (a–f, i–p) Bar = 50 μm, (g, h) bar = 500 μm.

Article Snippet: A human melanoma tissue microarray (TMA) containing 37 evaluable cores annotated according to primary vs metastatic melanoma origin and with survival outcomes was evaluated (Imgenex, San Diego, CA, USA).

Techniques: Microarray, Expressing, Immunostaining